ASSESSMENT OF HEMOSTATIC ACTIVITY OF THE AQUEOUS EXTRACT OF LEAVES OF MARRUBIUM VULGARE L, A MEDITERRANEAN LAMIACEAE ALGERIA

Authors

  • Nabil Ghedadba Laboratory of Materials Chemistry and living: Activity and Reactivity. Department of natural science and life, Faculty of Science, El- Hadj- Lakhdar University 05000. Batna, Algeria
  • Leila Hambaba Laboratory of Materials Chemistry and living: Activity and Reactivity. Department of natural science and life, Faculty of Science, El- Hadj- Lakhdar University. 05000. Batna, Algeria
  • Nassima Fercha Laboratory of Materials Chemistry and living: Activity and Reactivity. Department of natural science and life, Faculty of Science, El- Hadj- Lakhdar University. 05000. Batna, Algeria
  • Bousselsela Houas Laboratory of Materials Chemistry and living: Activity and Reactivity. Department of natural science and life, Faculty of Science, El- Hadj- Lakhdar University. 05000. Batna, Algeria
  • Samira Abdessemed Laboratory of Materials Chemistry and Living: Activity and Reactivity. Department of natural science and life, Faculty of Science, El-Hadj-Lakhdar University. 05000. Batna, Algeria
  • Sidi Mohamed Ould Mokhtar Unité de Biodiversité et Valorisation des Ressources Végétales (BVRV) – Faculté des Sciences et Techniques (FST)- Université des Sciences, de Technologie et de Médecine (USTM) Nouakchott, Mauritanie

DOI:

https://doi.org/10.20319/lijhls.2016.s21.253258

Keywords:

Marrubium Vulgare L, Aqueous Extract, Phenolic Compounds Dosing, Hemostatic Activity, Condensed Tannins

Abstract

The overall objective of this study was to evaluate in vitro the hemostatic activity of secondary metabolites (polyphones, flavonoïds and tannins) of Marrubium vulgare leaves, aromatic plant widely used in traditional medicine for the treatment of asthma, cough, diabetes ( by its effect on the pancreas to secrete insulin), heart disease, fever has a high efficiency as against inflammation. Qualitative analysis of the aqueous extract (AQE ) by thin layer chromatography revealed the presence of quercetin, kaempferol and rutin. Quantification of total phenols by Folin Ciocalteu method and flavonoïds by AlCl3 method gave high values with AQE: 175 ± 0.80 mg GAE per 100g of the dry matter, 23.86 ± 0.36 mg QE per 100g of dry matter. Moreover, the assay of condensed tannins by the vanillin method showed that AQE contains the highest value: 16.55 ± 0.03 mg E-Catechin per 100g of dry matter. Assessment of hemostatic activity by the plasma recalcification method (time of Howell) has allowed us to discover the surprising dose dependent anticoagulant effect of AQE lyophilized from leaves of M. vulgare. A positive linear correlation between the two parameters studied: the content of condensed tannins and hemostatic activity (r = 0.96 ) were used to highlight a possible role of these compounds that are potent vasoconstrictor activity in hemostatic. From these results we can see that Marrubium vulgre could be used for the treatment of health.

References

Aouissa, I. W. R. (2002). Etude des activités biologiques et de la toxicité aigüe de l’extrait aqueux des feuilles de Mangifera indica L. (Anacardiacées). Thèse de pharmacie. Bamako: Université de Bamako, 127.

El-Bardai, S., Lyoussi, B., Wibo, M., & Morel, N. (2004). Comparative study of the antihypertensive activity of Marrubium vulgare and of the dihydropyridine calcium antagonist amlodipine in spontaneously hypertensive rat. Clin Experiment Hypertension, 26, 465–74. http://dx.doi.org/10.1081/CEH-200031818

Elberry, A. A., Harraz, F. M., Ghareib, S. A., & al. (2011). Methanolic extract of Marrubium vulgare ameliorates hyperglycemia and dyslipidemia in Streptozotocin-induced diabetic rats. [Special issue]. Int J Diabetes Mellit, doi:10.1016/j.ijdm. 2011.01 .004*ht tp://dx.doi.org/10.1016/j.ijdm.2011.01.004

Ghedadba, N., Bousselsela, H., Hambaba, L., Benbia S., & Mouloud, Y. (2014). Évaluation de l’activité antioxydante et antimicrobienne des feuilles et des sommités fleuries de Marrubium vulgare L. Phytothérapie, 12, 15-24. http://dx.doi.org/10.1007/s10298-014-0832-z

Orhan, E., Belhattab, R., Senol, F. S., Gülpinar, A. R., Hosbas, S., & Kartal, M. (2010). Profiling of cholinesterase inhibitory and antioxidant activities of Artemisia absinthium, A. herba-alba, A. fragrans, Marrubium vulgare, M. astranicum, Origanum vulgare subsp. glandulossum and essential oil analysis of two Artemisia species. Industrial Crops and Products, 32, 566-571. http://dx.doi.org/10.1016/j.indcrop.2010.07.005

Rigano, D., Aviello, G., Bruno, M., Formisano, C., Rosselli, S., Capasso, R., Senatore, F., Izzo, A. A., & Borrelli, F. (2009). Antispasmodic effects and structure-activity relationships of labdane diterpenoids from Marrubium globosum ssp. Libanoticum, J. Nat. Prod, 72, 1477–1481. http://dx.doi.org/10.1021/np9002756

Stulzer, H. K., Tagliari, M. P., Zampirolo, J. A., Cechinel-Filho, V., & Schlemper, V. (2006). Antioedematogenic effect of marrubiin obtained from Marrubium vulgare. Journal of Ethnopharmacology, 108 (3), 379-384. http://dx.doi.org/10.1016/j.jep.2006.05.023

Wojdylo, A., Oszmianski, J., & Czemerys, R. (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem, 105, 940–949. http://dx.doi .org/1 0.10 16/j.foodchem.2007.04.038

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Published

2016-01-01

How to Cite

Ghedadba, N., Hambaba, L., Fercha, N., Houas, B., Abdessemed, S., & Mokhtar, S. M. O. (2016). ASSESSMENT OF HEMOSTATIC ACTIVITY OF THE AQUEOUS EXTRACT OF LEAVES OF MARRUBIUM VULGARE L, A MEDITERRANEAN LAMIACEAE ALGERIA. LIFE: International Journal of Health and Life-Sciences, 2(1), 253–258. https://doi.org/10.20319/lijhls.2016.s21.253258