COMPARISON OF GC-MS INTRODUCING TECHNIQUES FOR 2-PHENYLETHANOL DETECTION FROM YEAST
DOI:
https://doi.org/10.20319/stra.2026.2740Keywords:
2-Phenylethanol, Gas Chromatography-Mass Spectrometry, Introducing Techniques, Direct Thermal DesorptionAbstract
2-Phenylethanol (2-PE) can be produced by microorganisms, especially Saccharomyces cerevisiae. Gas Chromatography-Mass Spectrometer (GC-MS) has been used to analyze volatile producing microorganisms. The Gas Syringe-Static Headspace (GS-SHS), Thin Film-Solid Phase Micro Extraction (TF-SPME), Headspace Sorptive Extraction (HSSE), Dynamic Headspace (DHS), and Direct Thermal Desorption (DTD) were used to introduce Saccharomyces cerevisiae LV-007 cultured medium to GC-MS for 2-PE qualitative and quantitative analyses. The numbers of detected volatiles from introducing techniques of GS-SHS, TF-SPME, HSSE, DHS, and DTD were 8, 26, 15, 27, and 16, respectively by qualitative analysis. After 2-PE and viable cell quantifications from LV-007 of various introducing techniques, DTD showed the highest 2-PE ratio of 237.37´10-6 μg/ml·CFU. The DTD technique also used short time of 30 min to analyze sample.
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